Platelet-Activating Factor Induces Th17 Cell Differentiation

Figure 3

PAF-stimulated LC induce a Th17 phenotype in cocultured T cells. Monocyte-derived LC were stimulated with graded concentrations of PAF or its vehicle (ethanol; V) and cocultured with antiCD3/CD28-activated CD4⁺ T cells for 5 days. RORγt (a) and IL-17 (b) expression was measured by FACS in CD4⁺ T cells. For comparison, CD4⁺ T cells were cultured alone with IL-1β, IL-6, and IL-23 for 5 days. Data (means ± SEM) are expressed as fold induction relative to vehicle (V) control. 𝑛=10; *𝑃<0.05; **𝑃<0.01; ***𝑃<0.001. (c) LC were also stimulated with either vehicle or PAF in the absence or presence of the PAFR antagonist WEB 2086 (10⁻⁵ M) and cocultured with anti-CD3/CD28-activated CD4⁺ T cells for 5 days. RORγt expression was measured by FACS in CD4⁺ T cells and expressed as geometric mean (±SEM) fluorescence intensity (MFI). 𝑛=5; **𝑃<0.01; ***𝑃<0.001.

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