Review Article

Dendritic Cells in Human Atherosclerosis: From Circulation to Atherosclerotic Plaques

Table 1

Immunohistochemical markers to identify DCs in human plaques.

MarkerDC typeReferencesPitfallsReferences

Fascin (p55)Immature/mature DCs; DC specific in early plaque stages [7, 59, 62, 65, 66, 68, 69, 7377]Capillary ECs, migrating vascular cells in plaque shoulders and advanced plaques [69, 77]
S100 (S100B and weakly S100A1)Immature/mature DCs; DC specific in normal intima and all plaque stages [7, 7, 56, 59, 61, 65, 68, 69, 7779]Nerve bundles and twigs in adventitia [7, 56, 59, 69]
LangerinSelectively expressed on the surface and in Birbeck granules of Langerhans cells [60, 69]Very few cells [60, 69]
CD1aMature DCs [68, 74, 78, 80]CD14+, CD68+ foam cells [69, 81]
CD83Mature DCs [6567]Aspecific staining due to signal amplification? Activated T cells and monocytes? [63, 69]
DC-SIGN (CD209)Immature/mature DCs [63, 64]Macrophages [63]
DC-LAMP (CD208)Mature DCs [65]
BDCA-1 (CD1c)mDC [26, 68, 69]B cells [23]
BDCA-2 (CD303)pDC precursor [26, 68, 69] [23, 38]
CD11cmDCs [7173]CD14+ monocytes and CD68+ macrophages [69]
CD123pDCs [7073]ECs, microvessels in advanced plaques, and plaque shoulders [69]

DC: dendritic cells, EC: endothelial cell, pDC: plasmacytoid DC, mDC: myeloid DC.