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Mediators of Inflammation
Volume 2012 (2012), Article ID 148039, 7 pages
http://dx.doi.org/10.1155/2012/148039
Review Article

Proteomic Analyses of the Vitreous Humour

1Liverpool Ocular Oncology Research Group, Department of Molecular and Clinical Cancer Medicine, Institute of Translational Medicine, University of Liverpool, Liverpool, UK
2Department of Ophthalmology, Spedali Civili di Brescia, University of Brescia, Brescia, Italy
3Department of Ophthalmology, Istituto Clinico Humanitas, Milan, Italy

Received 7 July 2012; Accepted 3 August 2012

Academic Editor: Ahmed M. Abu El-Asrar

Copyright © 2012 Martina Angi et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

The human vitreous humour (VH) is a transparent, highly hydrated gel, which occupies the posterior segment of the eye between the lens and the retina. Physiological and pathological conditions of the retina are reflected in the protein composition of the VH, which can be sampled as part of routine surgical procedures. Historically, many studies have investigated levels of individual proteins in VH from healthy and diseased eyes. In the last decade, proteomics analyses have been performed to characterise the proteome of the human VH and explore networks of functionally related proteins, providing insight into the aetiology of diabetic retinopathy and proliferative vitreoretinopathy. Recent proteomic studies on the VH from animal models of autoimmune uveitis have identified new signalling pathways associated to autoimmune triggers and intravitreal inflammation. This paper aims to guide biological scientists through the different proteomic techniques that have been used to analyse the VH and present future perspectives for the study of intravitreal inflammation using proteomic analyses.