Deoxyribonuclease Is a Potential Counter Regulator of Aberrant Neutrophil Extracellular Traps Formation after Major Trauma
Figure 4
Degradation of NETs by DNase in vitro. (a) Degradation of NETs in a concentration-dependent manner. Neutrophils were isolated from healthy volunteers () and stimulated 3 h with 50 nM PMA. Then, the supernatants were incubated with 0, 0.02, 0.2, 2.0, and 20.0 μg/mL rhDNase for 30 minutes and values of cfDNA/NETs were determined (n.d.: not detected). Data are presented as mean ± SEM. (b) Immunofluorescence staining of NETs. Neutrophils were stimulated with 30 nM PMA for 3 h and stained for DNA (blue) and MPO (green). Cells were further incubated with 20 μg/mL rhDNase.
