Zerumbone Attenuates the Severity of Acute Necrotizing Pancreatitis and Pancreatitis-Induced Hepatic Injury

Table 1

Experimental design of the preliminary research.

Group	n	Time
		−0.5h	0h	12 h
Sham-operated group	8	Vehicle (10% DMSO)	Saline	Sacrifice
Acute necrotizing pancreatitis group	8	Vehicle (10% DMSO)	Taurocholate	Sacrifice
Zerumbone pretreated group 5 mg/kg	8	Zerumbone 5 mg/kg	Taurocholate	Sacrifice
Zerumbone pretreated group 10 mg/kg	8	Zerumbone 10 mg/kg	Taurocholate	Sacrifice
Zerumbone pretreated group 20 mg/kg	8	Zerumbone 20 mg/kg	Taurocholate	Sacrifice
Zerumbone pretreated group 40 mg/kg	8	Zerumbone 40 mg/kg	Taurocholate	Sacrifice

In acute necrotizing pancreatitis group, rats received the vehicle (10% DMSO, 2 mL/kg) administered via femoral vein half an hour prior to retrograde infusion of a freshly prepared 5% sodium taurocholate solution (1 mg/kg). In sham-operated group, rats received the vehicle administered via femoral vein and after half an hour, the rats were retrograde infused isotonic saline solution (1 mL/kg). In zerumbone pretreated group, rats received 5, 10, 20, and 40 mg/kg zerumbone dissolved in vehicle (10% DMSO v/v) administered via femoral vein half an hour prior to infusion 5% sodium taurocholate solution (1 mg/kg).