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Mediators of Inflammation
Volume 2012 (2012), Article ID 174764, 7 pages
http://dx.doi.org/10.1155/2012/174764
Research Article

IL-17 and IL-11 GCF Levels in Aggressive and Chronic Periodontitis Patients: Relation to PCR Bacterial Detection

1Department of Medical Biochemistry & Molecular Biology, Faculty of Medicine, Cairo University, Egypt
2Department of Oral Medicine, Periodontology and Diagnosis, Faculty of Oral and Dental Medicine, Cairo University, Egypt

Received 24 July 2012; Revised 28 September 2012; Accepted 15 October 2012

Academic Editor: Hidde Bult

Copyright © 2012 Olfat G. Shaker and Noha A. Ghallab. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Objectives. This study evaluated IL-17 and IL-11 in gingival crevicular fluid (GCF) of generalized chronic periodontitis (GCP) and generalized aggressive periodontitis (GAgP) patients in relation to periodontopathic bacteria. Subjects and Methods. GCF samples were collected from 65 subjects including 25 CP, 25 GAgP, and 15 controls (C) and analyzed for IL-17 and IL-11 by an enzyme-linked immunosorbent assay. Molecular detection of bacteria in the dental plaque was determined by polymerase chain reaction. Results. The total amount of IL-17 was significantly higher in GAgP group than in GCP and C groups . The IL-11 concentration was significantly higher in C and GCP groups than GAgP group . The IL-11/IL-17 ratio was significantly higher in the C group than in GCP and GAgP groups . Moreover, GAgP group showed lower ratios of IL-11/IL-17 when compared to GCP group. The high positivity of P. gingivalis in the dental plaque was associated with significantly increased GCF levels of IL-17 in GCP and GAgP patients. Conclusions. The increased IL-17 level in GCF of GAgP suggests a potential role in the aetiopathogenesis. Meanwhile, the decreased ratio of IL-11/IL-17 might reflect an imbalance between the proinflammatory and anti-inflammatory cytokines in different periodontal diseases.