Activation of Human Neutrophils by the Anti-Inflammatory Mediator Esenbeckia leiocarpa Leads to Atypical Apoptosis
Figure 1
CHE induces actin filament polymerization in human PMNs. Freshly isolated human neutrophils were isolated and incubated (10 × 106 cells/mL) with the diluent (HBSS-1% DMSO, Ctrl), crude hydroalcoholic extract (CHE) (500 μg/mL), or fMLP (10−8 M) for 5, 15, or 30 min. Actin polymerization was evaluated by monitoring FITC-phalloidin expression by flow cytometry. (a) Results are means ± SEM (). (b) Results shown are from one representative experiments plotted in (a). * (versus Ctrl) by Student’s t-test.
