Activation of Human Neutrophils by the Anti-Inflammatory Mediator Esenbeckia leiocarpa Leads to Atypical Apoptosis
Figure 4
The pro-apoptotic activity of CHE does not correlate with CD16 shedding. Cells (10 × 106 cells/mL) were incubated with the diluent (SA), 1000 ng/mL Viscum album agglutinin-I (VAA), or 500 μg/mL CHE for 22 h and apoptosis was evaluated in parallel for each blood donors by cytology (a) or by monitoring CD16 cell surface expression by flow cytometry (b). Inset in (b): typical results obtained by FACS analysis and plotted in the corresponding bar graph. Results are means ± SEM (). * versus SA by Student’s t-test.