Human Mast Cells (HMC-1 5C6) Enhance Interleukin-6 Production by Quiescent and Lipopolysaccharide-Stimulated Human Coronary Artery Endothelial Cells
Figure 5
Histamine and Tryptase induce potentiation of IL-6 production by LPS-stimulated endothelial cells. Endothelial cells (10,000 cells/well) were incubated with histamine (10 μM) (a and b) or human tryptase (50 mU/mL) (c) in the presence or absence of LPS (100 ng/ml) for 24 h. After the incubation, culture supernatants were analyzed for IL-6 by ELISA. To determine the effect of H1R or H2R antagonists, endothelial cells were incubated with 10 μM diphenhydramine (DPH) (a) or 10 μM famotidine (FAM) (b) for 30 min before addition of histamine. Results are mean ± SEM of quadruplicate determinations of a representative experiment. Similar results were observed in two other experiments.
