Human Mast Cells (HMC-1 5C6) Enhance Interleukin-6 Production by Quiescent and Lipopolysaccharide-Stimulated Human Coronary Artery Endothelial Cells

Table 1

HMC-1 5C6 directly and synergistically with LPS enhance endothelial cell production of IL-6.

Treatment	No LPS	LPS
Control	25±3	546±19
5C6 (7,500)	82±6	1548±68
5C6 (15,000)	170±9	2238±59
5C6-sonicate (7,500 cell equivalent)	118±14	1380±45
5C6-sonicate (15,000 cell equivalent)	227±13	1842±62

Confluent human coronary artery endothelial cells (15,000 cells/well) were incubated with the indicated number of human mast cells or their lysate after sonication in the presence or absence of LPS (100 ng/mL). After 20 h of incubation the culture supernatants were assayed for IL-6. HMC-1 5C6 cultured in the absence of HCAEC did generate IL-6 in the presence or absence of LPS. Each value presented is the mean±SEM of quadruplicate determinations.