Figure 2: Rosiglitazone inhibits Poly (I:C)-induced HMGB1 release in RAW 264.7 cells. (a) Cells grown to 60% confluency were incubated with serum-free medium for 24 h and then stimulated with LPS and/or Poly (I:C) in the presence or absence of rosiglitazone for 24 h. Equal volumes of conditioned media were subjected to Western blot analysis for the detection of HMGB1. Ponceau S staining was used as a loading control. (b) At the same time, total protein was extracted, fractioned by electrophoresis, and immunoblotted with the indicated antibodies. The results shown are representative of three independent experiments.