Figure 3: Administration of rosiglitazone post-LPS treatment also attenuates LPS-induced release of HMGB1 in RAW 264.7 cells. Cells were grown to 60% confluency, incubated with serum-free medium for 24 h, and then treated with LPS. Rosiglitazone was administered at the indicated time points post-LPS treatment for 24 h. Conditioned medium was subjected to Western blot analysis for the determination of HMGB1 levels. Ponceau S staining was used as a loading control. The results shown are representative of three independent experiments.