Activation of Peroxisome Proliferator-Activated Receptor 𝛾 by Rosiglitazone Inhibits Lipopolysaccharide-Induced Release of High Mobility Group Box 1

Figure 5

Rosiglitazone inhibits LPS-induced TLR4 signal pathway in RAW 264.7 cells. (a) Cells incubated with serum-free medium for 24 h were treated with LPS in the presence or absence of rosiglitazone for 6 h. The mRNA levels of indicated genes were determined by real-time PCR using SYBR Green. GAPDH was used as an internal standard. (b) Cells were incubated in serum-free medium for 24 h and then stimulated with LPS in the presence or absence of rosiglitazone for 9 h. Total protein was extracted, fractionated by electrophoresis, and immunoblotted with the indicated antibodies. (c) Cells incubated with serum-free medium for 24 h were treated with LPS in the presence or absence of rosiglitazone for 1 h. An aliquot of protein was immunoblotted with phospho-I𝜅B𝛼 (p-I𝜅B𝛼) and 𝛽-actin antibodies. The results are expressed as the means ± S.E. (𝑛=3). **𝑃<0.01 compared to untreated group; ^#𝑃<0.05, ^##𝑃<0.01 compared to LPS-treated group.