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Mediators of Inflammation
Volume 2012 (2012), Article ID 398207, 9 pages
http://dx.doi.org/10.1155/2012/398207
Research Article

Disruption of the ECM33 Gene in Candida albicans Prevents Biofilm Formation, Engineered Human Oral Mucosa Tissue Damage and Gingival Cell Necrosis/Apoptosis

1Groupe de Recherche en Écologie Buccale, Faculté de Médecine Dentaire, Université Laval Pavillon de Médecine Dentaire, 2420 Rue de la Terrasse, QC, Canada G1V 0A6
2Center for Medical Mycology, Department of Dermatology, School of Medicine, Case Western Reserve University and University Hospitals Case Medical Center, Cleveland, OH 44106, USA

Received 13 December 2011; Revised 3 March 2012; Accepted 8 March 2012

Academic Editor: Teresa Zelante

Copyright © 2012 Mahmoud Rouabhia et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

In this study we demonstrated that ΔCaecm33 double mutant showed reduced biofilm formation and causes less damage to gingival mucosa tissues. This was confirmed by the reduced level of necrotic cells and Bax/Bcl2 gene expression as apoptotic markers. In contrast, parental and Caecm33 mutant strains decreased basement membrane protein production (laminin 5 and type IV collagen). We thus propose that ECM33 gene/protein represents a novel target for the prevention and treatment of infections caused by Candida.