Cathepsin G Induces Cell Aggregation of Human Breast Cancer MCF-7 Cells via a 2-Step Mechanism: Catalytic Site-Independent Binding to the Cell Surface and Enzymatic Activity-Dependent Induction of the Cell Aggregation
Figure 1
MCF-7 cell aggregation-inducing activities of cathepsin G (CG) and chymotrypsin. (a) MCF-7 cell aggregation assay using CG and chymotrypsin. MCF-7 cells ( cells/well) were seeded in 96-well plates in RPMI 1640 medium containing 5% fatal bovine serum (FBS). The cells were cultured overnight and then incubated overnight with the serine proteases in RPMI 1640 medium containing 1% BSA. After washing, the residual cells were stained with crystal violet, and the aggregation index was calculated as described in Section 2. The results are expressed as mean SD . When the bars are not shown, they are smaller than the size of the symbols. (b) Images of MCF-7 cells at 24 h after incubation with the serine proteases. Scale bar = 50 m.
