Lipid Bodies: Inflammatory Organelles Implicated in Host-Trypanosoma cruzi Interplay during Innate Immune Responses
Figure 3
Trypanosoma cruzi infection induces macrophage lipid body formation. Peritoneal macrophages isolated from mice were cultured or not with T. cruzi and the formation of lipid bodies analyzed after 24 h by staining with BODIPY 493/503 (a and b), a fluorescent lipid probe for highly hydrophobic environments [35] or osmium tetroxide (c and d). While uninfected cells (a, c) have small number of lipid bodies, infected cells (b and d) show increased number of these organelles. Lipid bodies are seen as green (a and b) or brownish (c and d), round organelles. Nuclei of macrophages and internalized parasites (arrows) were stained with DAPI (4′,6-diamidino-2-phenylindole; blue). Scale bar, 10 μm.
