Low Dose Theophylline Showed an Inhibitory Effect on the Production of IL-6 and IL-8 in Primary Lung Fibroblast from Patients with COPD

Figure 1

Increased mRNA expression and protein production of interleukin-6 (IL-6) in lung fibroblasts from COPD patients. (a) Real-time RT-PCR was adopted to evaluate the transcription levels of IL-6, and the relative expression was calculated based on the expression of GAPDH. ***𝑃<0.001, when compared to control group. (b) The relationship of IL-6 mRNA expression and FEV₁. (c) The protein level of IL-6. ELISA was used to measure the concentration of IL-6 in the cell-free supernatants. *𝑃<0.05, when compared to control group. (d) The relationship of IL-6 secretion and FEV₁.

(a)

(b)

(c)

(d)