Altered AKT1 and MAPK1 Gene Expression on Peripheral Blood Mononuclear Cells and Correlation with T-Helper-Transcription Factors in Systemic Lupus Erythematosus Patients
Table 2
Primers for quantitative RT-PCR.
Gene1
Accession no.1
Description
SABiosciences Cat. no.2
IL-1B
NM_000576
Interleukin 1, beta
PPH00171B
IFNG
NM_000619
Interferon, gamma
PPH00380B
IL-12A
NM_000882
Interleukin 12A
PPH00544B
IL-6
NM_000600
Interleukin 6
PPH00560B
TNF-alpha
NM_000594
Tumor necrosis factor (TNF superfamily, member 2)
PPH00341E
IL-10
NM_000572
Interleukin 10
PPH00572B
IL-2
NM_000586
Interleukin 2
PPH00172B
TGFB2
NM_003238
Transforming growth factor, beta 2
PPH00524B
IL-5
NM_000879
Interleukin 5
PPH00692A
TNFSF10
NM_003810
Tumor necrosis factor (ligand) superfamily, member 10
PPH00242E
GAPDH
NM_002046
Glyceraldehyde-3-phosphate dehydrogenase
PPH00150E
ACTB
NM_001101
Actin, beta
PPH00073E
TBX21
NM_013351
T-box-21 (T-bet)
PPH00396A
GATA3
NM_002051
GATA-binding protein 3
PPH02143A
FOXP3
NM_014009
Forkhead box P3
PPH00029B
STAT4
NM_003151
Signal transducer and activator of transcription 4
PPH00777E
RORC
NM_005060
RAR-related orphan receptor C
PPH05877A
MAPK1
NM_002745
Mitogen-activated-protein-kinase 1
PPH00715B
AKT1
NM_005163
v-akt murine-thymoma viral-oncogene-homolog 1
PPH00088A
CD38
NM_001775
CD38 molecule
PPH00856A
ZAP70
NM_001079
Zeta-chain (TCR) associated protein kinase 70 kDa
PPH00256A
CD247
NM_000734
CD247 molecule, CD3-zeta.
PPH01484A
CCR6
NM_004367
Chemokine (C-C motif) receptor 6
PPH00616E
Gene1,3
Forward primer (5′–3′)4
Reverse Primer (5′–3′)4
Ref.
GAPDH (NM_002046)
gaaggtgaaggtcggagtc
gaagatggtgatgggatttc
Primer3 software3
1http://www.ncbi.nlm.nih.gov/
.
2SABiosciences, QIAGEN company.
3http://frodo.wi.mit.edu/primer3/
.
4Primers used for this study based on the literature were computationally checked for sequence specificity. Melt-curve analysis for each primer pair and reaction was also tested to verified specific amplification.
