Altered AKT1 and MAPK1 Gene Expression on Peripheral Blood Mononuclear Cells and Correlation with T-Helper-Transcription Factors in Systemic Lupus Erythematosus Patients

Table 4

Cytokines plasma levels.

Cytokines^a, b	SLE (n = 13)	Controls (n = 13)	P value^c
IL-1β	0.24 (0.10–0.96)	0.10 (0.10–0.13)	0.0112
IL-2	0.61 (0.60–20.24)	0.60 (0.60–2.62)	0.1893
IL-4	0.10 (0.10–0.68)	0.10 (0.10–0.19)	0.6607
IL-5	1.00 (1.00–1.00)	0.90 (0.80–1.00)	0.0077
IL-6	1.00 (1.00–22.35)	1.00 (0.85–1.00)	0.0117
IL-10	2.82 (0.75–9.64)	0.63 (0.60–3.22)	0.1368
IL-12(p70)	1.02 (0.60–1.68)	0.18 (0.06–0.50)	0.0009
IL-13	0.20 (0.20–3.87)	0.20 (0.19–0.21)	0.0685
IFN-γ	0.64 (0.20–3.76)	0.20 (0.20–0.33)	0.0445
TNF-α	0.34 (0.10–2.10)	0.10 (0.10–0.30)	0.1193

^aA multiplex bead array (Bio-Plex, BioRad) was used to simultaneously measure 10 different cytokines in each plasma sample. Samples from patients and controls were analyzed in parallel. Values are in pg/mL. Limits of detection (pg/mL) for the indicated cytokines were: 0.1, 0.64, 0.17, 0.88, 0.29, 0.17, 0.37, 0.19, 0.35, and 0.2, respectively. Analyses of data were performed using five-parameter logistic curve fitting to standard analyte values. Intra-assay and interassay CV were ≤8% and ≤10%, respectively.
^bValues are presented as Median (25th to 75th percentiles).
^cMann-Whitney U test for comparison between SLE and controls.
Significance levels set at P values <0.05. The analysis of the data was done using the GraphPad Prism v.5.01 software.