Immunophenotyping of Posttraumatic Neutrophils on a Routine Haematology Analyser
Figure 2
Agreement between two flow cytometers. Samples of whole blood of ten healthy volunteers were measured on both a FACSCalibur and a Cell-Dyn Sapphire to demonstrate the agreement between median fluorescent intensity (MFI) of monoclonal antibodies on neutrophils. Whole blood was incubated with (a) RPE-labeled CD11b (MAC-1); (b) FITC-labeled CD62L (L-selectin); (c) Alexa Fluor 647-labeled CD16 (FcγRIII) for the experiment on the CD-Sapphire and ECD-labeled CD16 (FcγRIII) for the experiment on the FACSCalibur; (d) FITC-labeled MhoPhab A27, a monoclonal phage antibody, which recognizes active FcγRII (CD32). The solid lines are regression lines. = Spearmans rank correlation coefficient. ns = Significant.
