Anti-Inflammatory Properties of Sirtuin 6 in Human Umbilical Vein Endothelial Cells
Figure 3
Effect of SIRT6 knockdown on proinflammatory cytokines. HUVECs were transfected with 50 nM SIRT6 or NS siRNA for 72 h followed by 10 g/mL LPS for 24 h (). (a) IL-6 and IL-8 concentration in the conditioned media was assayed using ELISA. (b) IL-1, IL-6, and IL-8 mRNA expression was quantified by qRT-PCR. Each bar represents the mean SEM. versus NS siRNA + LPS.
