Apoptotic Cells Contribute to Melanoma Progression and This Effect is Partially Mediated by the Platelet-Activating Factor Receptor
Figure 3
Treatment with PAFR antagonist reduces neutrophil and macrophage infiltration. The inhibition of PAFR using PCA4248 (PCA) results in reduced neutrophil and macrophage infiltration in mice submitted to coinjection of apoptotic cells (106γ-irradiated Tm1) and a subtumorigenic dose of Tm1 melanoma cells (103 viable cells). Subcutaneous tissues from animals treated with PBS or PCA4248 (PCA), for 5 consecutive days, were surgically removed 6, 24 and 72 h after coinjection and processed for immunohistochemistry analysis using specific antibodies against myeloperoxidase (antigen expressed mainly by neutrophils, (a)) and F4/80 (cell marker by macrophages, (b)). *Denotes statistical significance () using nonpaired nonparametric Student’s t-tests.
