Peripheral Leukocytapheresis Attenuates Acute Lung Injury Induced by Lipopolysaccharide In Vivo
Figure 4
The effect of LCAP on p65 protein levels in lung parenchyma, total protein concentration in BALF, wet/dry ratio in lung, and apoptotic changes in lung tissues. The p65 was examined by ELISA kits, and optical density was read at 450 nm (a). The water content of the tissues from left inferior lung was shown as wet/dry weight ratio (b). The total protein concentration in BALF assayed by methods of Bradford (c); apoptotic cells (arrow) in left inferior lung parenchyma were detected by TUNEL in situ assay using an In Situ Cell Death Detection Kit (d); incidence rate of ALI/ARDS was diagnosed according to the criteria for ALI/ARDS by the American-European Consensus Conference of 1994 (e). The mean levels of PMN counts and NE in the 7 animals without ALI were compared to 17 animals with ALI (f). *indicates significant difference among the LCAP, LPS, and the sham-LCAP groups (*; **); indicates significant difference among the LCAP, LPS, and sham-LCAP groups ().
