CRP and TNF-Induce PAPP-A Expression in Human Peripheral Blood Mononuclear Cells
Figure 4
Effect of BAY11-7082 on cytokine-induced PAPP-A expression. PBMCs were pretreated with BAY11-7082 (20 μM) for 60 min and then treated with CRP (20 mg/L) or TNF-α (100 ng/mL) for 24 hours. (a) Detection of PAPP-A mRNA expression was conducted by RT-PCR. (b) PAPP-A protein expression were determined by Western blotting. (c) PAPP-A concentrations were performed by an ultra-sensitive ELISA. The results are expressed as the means ± SD of six donors. *: compared with the control; #: the CRP group versus the CRP + BAY group; ▲: the TNF-α group versus the TNF-α + BAY group.