Superoxide Dismutase 3 Limits Collagen-Induced Arthritis in the Absence of Phagocyte Oxidative Burst
Figure 6
No oxidative burst was detected from the Ncf1 mutated mice. In vivo imaging with the ROS sensitive L-012 probe revealed a bright luminescent signal from the inflamed wild-type paws with CIA, while no signal was detected from Ncf1*/* mice with comparable arthritis severity (a). Imaging was reproduced in two independent experiments with similar results. Expression of SOD3 by Ade-SOD3 expression vector in the treated paws was confirmed by Q-RT-PCR (/group) (b). Ex vivo oxidative burst was measured from phagocytes from Ncf1*/* () and wild-type mice () by using DHR-123 assay with and without (w/o) PMA stimulation. Neither macrophages (c) nor neutrophils (d) from the Ncf1*/* mice were able to exert a measureable oxidative burst.
