Figure 3: Effects of enzyme inhibitors on RGAP- or LPS-mediated NO production in RAW264.7 cells. (a and b) Levels of NO were determined by the Griess assay from culture supernatants of RAW264.7 cells pretreated with MAPK inhibitors (U0126 (U0), SB203580 (SB), and SP600125 (SP)), tyrosine kinase inhibitors (PP2, piceatannol (Pic), and AG126 (AG)), LY294002 (LY), and BAY11-7082 (BAY), after RGAP (1 mg/mL) or LPS (1 μg/mL) treatment for 24 h. * and ** compared to control.