Figure 4: Effect of RGAP and LPS on the activation of upstream signalling enzymes for NF-κB and AP-1 translocation. (a and b) Phosphorylated or total protein levels of IκBα, Akt, ERK, JNK, p38, and β-actin from cell lysates prepared with RGAP- or LPS-treated RAW264.7 cells were determined by phosphospecific or total protein antibodies.