732860.fig.005
Figure 5: Effects of blocking antibodies on RGAP-mediated NO production in RAW264.7 cells. Levels of NO were determined by the Griess assay from culture supernatants of RAW264.7 cells pretreated with blocking antibodies to TLR2, TLR4, and dectin-1 2 h before stimulation with RGAP, pam3CSK, β-glucan, or LPS (1 μg/mL) treatment for 24 h. * and ** compared to control.