Figure 6: Effects of RGS2 and wortmannin on RGAP-mediated NO production in peritoneal macrophages. Levels of NO were determined by the Griess assay from culture supernatants of peritoneal macrophages prepared from wild-type or RGS2 knockout mice in the presence or absence of wortmannin, stimulated with RGAP or LPS (1 μg/mL) for 24 h. * and ** compared to control.