CXCL1-Triggered Interaction of LFA1 and ICAM1 Control Glucose-Induced Leukocyte Recruitment during Inflammation In Vivo
Figure 5
Glucose-induced expression of ICAM1 in surgically prepared cremaster muscle venules. Immunostaining was conducted to assess endothelial expression of ICAM1 in postcapillary venules of cremaster muscles obtained 15 minutes after exteriorization and injection of 0.5 g/kg glucose or the equivalent volume of normal saline. Application of primary antibody was performed i.v. before harvesting the cremaster muscle in order to stain ICAM1 on the endothelial surface. Biotinylated secondary antibody, peroxidase-conjugated streptavidin, and diaminobenzidine (DAB) were used to detect endothelial expression of ICAM1 as brown signal. Counter-staining was performed by Mayer’s hemalaun. Intensity of venular anti-ICAM1 immunostaining during trauma-induced inflammation was analyzed semiquantitatively and presented as mean ± SEM (a; 0 = no, 1 = weak, 2 = medium, 3 = strong signal; at least 3 mice/group). In addition, representative images of saline- and glucose-treated mice are depicted in (b) and (c), respectively. Reference bar is shown in (b) and represents 30 μm. Significant differences are indicated by the asterisk.
