Biphasic Activation of Nuclear Factor-Kappa B in Experimental Models of Subarachnoid Hemorrhage In Vivo and In Vitro

Figure 5

The mRNA levels of NF-κB downstream genes were detected by real-time PCR in brain cortex after SAH. (a) IL-1β gene expression was significantly increasing after SAH (𝑃<0.05) and showed a biphasic increasing. Day 1, day 3, and day 14 groups showed the highest expression; (b) TNF-α gene showed biphasic expression in day 1, day 3, and day 14 groups; (c) caspase-3 gene significantly increased in day 3, day 6, and day 10 groups (𝑃<0.05), and the expression also showed two peaks on day 3 and day 10; (d) Bcl-2 gene expression was inhibited after SAH. In day 1 and day 3 groups, Bcl-2 was decreased significantly (𝑃<0.05), and in day 6 and day 14 groups, Bcl-2 was obviously increased (𝑃<0.05). *𝑃<0.05, **𝑃<0.01, and ***𝑃<0.001 versus control group. Bars represent the mean ± SEM (𝑛=4, each group).

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