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Mediators of Inflammation
Volume 2012, Article ID 809801, 7 pages
Research Article

Biochemical Analysis of Pentraxin 3 and Fibrinogen Levels in Experimental Periodontitis Model

1Department of Periodontology, Faculty of Dentistry, Ondokuzmayis University, 55139 Samsun, Turkey
2Department of Histology and Embryology, Faculty of Medicine, Ondokuzmayis University, 55139 Samsun, Turkey
3Department of Microbiology, Faculty of Veterinary Medicine, Ondokuzmayis University, 55139 Samsun, Turkey
4Department of Periodontology, Faculty of Dentistry, Istanbul Aydin University, 34380 Istanbul, Turkey

Received 22 March 2012; Revised 22 May 2012; Accepted 5 July 2012

Academic Editor: Hidde Bult

Copyright © 2012 Gonca Cayir Keles et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Objective. Pentraxin 3 (PTX3), newly discovered inflammation marker, is a member of acute-phase proteins. The hypothesis, synthesis of gingival tissue and serum PTX-3 increases in the experimental periodontitis model (with 10-day and 40-day periods), was tested by detecting gingival tissue and serum PTX-3 levels in rats with experimental periodontitis. Methods. Thirty rats were randomly divided into three groups of ten animals each: ligature-induced experimental periodontitis groups (with 10-day (Group1) and 40-day periods (Group2)) and healthy group (Group3). At the end of experimental period, rats were sacrificed, and radiological and histomorphometric analyses were performed on the mandibles. PTX3 levels were measured in gingival tissue and serum samples using ELISA. Plasma fibrinogen levels were measured according to the nephelometric method. Results. Significant alveolar bone resorption and periodontal inflammation were evident in periodontitis groups. Levels of PTX3 in gingival tissue were statistically higher in Group 1 than those in groups 2 and 3 ( ). No significant difference was found in serum PTX3 levels between experimental periodontitis and control groups ( ). Plasma fibrinogen levels were significantly increased in the experimental periodontitis groups ( ). Conclusion. PTX3 seems to be associated with tissue destruction in earlier periods of inflammatory periodontal disease, contrary to the fibrinogen findings.