The Impact of Various Reactive Oxygen Species on the Formation of Neutrophil Extracellular Traps
Figure 5
Assessment of intra- and extracellular ROS production of neutrophils after exposure to various inhibitors as measured by the luminol-amplified chemiluminescence assay. Freshly isolated human neutrophils were incubated with or without inhibitors (10 μM Rotenone, 10 μM Dinitrophenol, 500 nM FCCP, 20 μM DPI, 38 μM Aroclor, 10 μM DETC, 200 μM Dipyrone, and 200 μM Aminopyrine) for 30 min at 37°C. Subsequently, luminol was added, and the ROS production was induced with 20 nM PMA. The H2O2 release was detected by measuring chemiluminescence for a period of 1 h at 37°C. (a) Time kinetics of H2O2 release (chemiluminescence) of one representative experiment. (b) H2O2 release quantified by calculation of the area under the curve (AUC). Data show mean ± SD from three independent experiments, **: ; *: as compared to the PMA-stimulated sample without inhibitor (Medium).
