The Impact of Various Reactive Oxygen Species on the Formation of Neutrophil Extracellular Traps

Figure 7

Release of NETs by neutrophils after exposure to various ROS inhibitors assessed by fluorescent and scanning electron microscopy (SEM). Freshly isolated human neutrophils were preincubated with various ROS inhibitors (20 μM DPI, 38 μM Aroclor, and 200 μM Dipyrone) for 30 min at 37°C. NET release was induced by 20 nM PMA, and the samples were fixed after 4 h. NET release was detected by fluorescent microscopy and SEM. (a-b) DNA staining with SYTOXgreen and neutrophil elastase or (c–g) with SYTOXgreen alone. (a) and (c) unstimulated neutrophils, (b) and (d) PMA-stimulated neutrophils in medium (without inhibitor), (e–g) PMA-stimulated neutrophils exposed to a ROS inhibitor.