Mediators of Inflammation / 2012 / Article / Fig 1

Research Article

Adipose Tissue-Specific Deletion of 12/15-Lipoxygenase Protects Mice from the Consequences of a High-Fat Diet

Figure 1

The generation of a targeted conditional knockout allele for the mouse 12/15-lipoxygenase gene, ALOX15. (a) The targeted ALOX15 locus contains loxP sites that flank exons 2 and 5 and a neomycin (neo) cassette selection marker flanked by FRT sites inserted in intron 5. The neo cassette is driven by the mouse phosphoglucokinase gene (PGK) promoter and contains a polyadenylation (pA) signal to terminate the neomycin expression. Exons are depicted as blackened boxes, loxP sites as white triangles, and FRT sites as striped triangles. (b) 12/15-LO mRNA expression was measured in isolated epididymal adipocytes by RT-PCR. All data was normalized to total actin, and the fold changes in expression were calculated relative to control. All data represent the means SEM; = 3–6. and versus control. Chow: chow diet; HFD: high-fat diet; wt: wild type 12/15- . All measurements are performed on 24-week-old mice.