Calorie Restriction with a High-Fat Diet Effectively Attenuated Inflammatory Response and Oxidative Stress-Related Markers in Obese Tissues of the High Diet Fed Rats
Effect of HFCR on protein expression related to inflammation and oxidative stress in liver. (a) Western blot assay of pIκBα, iNOS, COX-2, Nrf2, and HO-1. (b) densitometric analysis of bands in Western blot. CON: rats fed a control diet (10% kcal fat) ad libitum for the experimental periods, HF: rats fed a high-fat diet (45% kcal fat) ad libitum for the experimental periods, and HFCR: 40% calorie restricted rats fed a high-fat diet for 8–10 weeks after being fed the high-fat diet ad libitum for 11–13 weeks. a.u.: arbitrary unit. Values are means ± SEM, in each. Means for a variable without a common letter differ, .