Anesthetic Isoflurane Posttreatment Attenuates Experimental Lung Injury by Inhibiting Inflammation and Apoptosis
Figure 5
HO-1 activation and iNOS suppression induced by 1.4% ISO treatment in ZY-stimulated lungs. Amplicons were densitometrically quantified, and the relative amounts of amplified HO-1 mRNA (a) and iNOS mRNA (e) were normalized against β-actin; Western blot analysis for expression of HO-1 (b) and iNOS (f); protein bands were densitometrically quantified and relative amounts of HO-1 protein (c) and iNOS protein (g) were normalized against β-actin; and detection of HO-1 (d) and iNOS (h) enzyme activities. The respective densitometric analysis of mRNA and protein bands is from three separate experiments. The animals were treated as described in Figure 1. Data are mean ± SD ( mice per group). * versus sham; # versus ZY + vehicle.
