Neisseria gonorrhoeae Induces a Tolerogenic Phenotype in Macrophages to Modulate Host Immunity
Figure 2
Cytokines induced in murine macrophage cell line infected with N. gonorrhoeae P9-17 LPS is the positive control. A statistically significant difference is found when data is compared to the level of cytokines found in the medium from cultures without infection. (a) Secretion of TNF-α by RAW cells measured 24 h after challenge. Data represent the levels of cytokine relative to levels induced by LPS. Bars are the mean SEM of 6 independent experiments. (b) Secretion of IL-10 after 24 h treatment. ** indicates that P9-17 induces higher levels of IL-10 than LPS and other treatments . (c) TGF-β1 levels on the surface of treated macrophages. Values correspond to the mean fluorescence intensity (G-mean) values relative to the levels found in untreated cells. Data are presented as the mean ± SEM of challenge experiments. * indicates that P9-17 induces higher levels of TGF-β1 than LPS and other treatments .
