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Mediators of Inflammation
Volume 2013 (2013), Article ID 152860, 11 pages
http://dx.doi.org/10.1155/2013/152860
Research Article

Expression of Annexin-A1 and Galectin-1 Anti-Inflammatory Proteins and mRNA in Chronic Gastritis and Gastric Cancer

1Department of Biology, São Paulo State University (UNESP), Campus de São José do Rio Preto, Rua Cristóvão Colombo 2265, 15054-000 São José do Rio Preto, SP, Brazil
2Rio Preto Endoscopy Center, São José do Rio Preto, SP, Brazil
3Endoscopy Service, Hospital de Base, São José do Rio Preto, SP, Brazil
4Legal Medicine Department and Pathology Service, Hospital de Base, São José do Rio Preto, SP, Brazil
5Institute of Anatomical Pathology and Cytopathology, São José do Rio Preto, SP, Brazil

Received 7 May 2012; Accepted 28 December 2012

Academic Editor: Eeva Moilanen

Copyright © 2013 Yvana Cristina Jorge et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Objective. The anti-inflammatory proteins annexin-A1 and galectin-1 have been associated with tumor progression. This scenario prompted us to investigate the relationship between the gene and protein expression of annexin-A1 (ANXA1/AnxA1) and galectin-1 (LGALS1/Gal-1) in an inflammatory gastric lesion as chronic gastritis (CG) and gastric adenocarcinoma (GA) and its association with H. pylori infection. Methods. We analyzed 40 samples of CG, 20 of GA, and 10 of normal mucosa (C) by the quantitative real-time PCR (qPCR) technique and the immunohistochemistry assay. Results. High ANXA1 mRNA expression levels were observed in 90% (36/40) of CG cases (mean relative quantification RQ =  ) and in 80% (16/20) of GA cases (mean RQ =  ). However, LGALS1 mRNA levels were high (mean RQ =  ) in 60% (12/20) of the GA cases, while low expression was found in CG (mean RQ =  ; ). Normal mucosa showed modest immunoreactivity in stroma but not in epithelium, while stroma and epithelium displayed an intense immunostaining in CG and GA for both proteins. Conclusion. These results have provided evidence that galectin-1 and mainly annexin-A1 are overexpressed in both gastritis and gastric cancer, suggesting a strong association of these proteins with chronic gastric inflammation and carcinogenesis.