Paraoxonase-1 Inhibits Oxidized Low-Density Lipoprotein-Induced Metabolic Alterations and Apoptosis in Endothelial Cells: A Nondirected Metabolomic Study

Figure 1

PON1 lactonase activity (a) and total peroxide concentrations (b) in the supernatant of the HUVEC cell culture (𝑛=3, for each experiment). Endothelial cells were incubated over 24 h with 50 mg/L isolated human LDL (L); 50 mg/L oxidized LDL (O); 50 mg/L oxidized LDL + 40 mg/L HDL from wild type mice (OH); 50 mg/L oxidized LDL + 40 mg/L HDL from PON1^(−/−) mice (KO); or with serum-free media as controls (C). ^∗𝑃<0.05, with respect to C; ^†𝑃<0.05, with respect to O; ^‡𝑃<0.01, with respect to C.

(a)

(b)