Induction of Tumor Necrosis Factor (TNF) Release from Subtypes of T Cells by Agonists of Proteinase Activated Receptors
Induction of TNF secretion from human purified T cells by thrombin and trypsin. Cells were incubated with (a) various concentrations of thrombin (Th, μg/mL), SFLLR (μM), TFLLRN (μM), and their reverse peptides RLLFS (μM), NRLLFT (μM), and (b) trypsin (Ts, μg/mL) in the presence or absence of their inhibitors, respectively, for 16 h at 37°C. Values shown are mean ± SEM for four to six independent experiments from different donors. * compared with the response to medium alone control. † compared with the response to the corresponding proteinase alone. Hir = hirudin (U/mL), TI = SBTI (μg/mL), and SCH = SCH 79797 (μM).