TNF-α and IFN-s-Dependent Muscle Decay Is Linked to NF-𝜿B- and STAT-1α-Stimulated Atrogin1 and MuRF1 Genes in C2C12 Myotubes

Figure 1

Short- and long term effects of TNF-α, IFNα, IFNγ (10 ng/mL each), insulin (10 nM), and metabolic inhibitors (at concentrations indicated) upon cell viability during 5 subsequent days of myogenic differentiation (a, b), cell mitogenicity determined at the 3rd day of experiment (c), NF-κB transcriptional activity in the 3rd day of myogenesis (d, e, h, i), cytosolic IκB and nuclear NF-κB expression levels upon TNF-α, and additional insulin pretreatment in the 3rd day of myogenesis (f, g). Actin was used as loading control. The results are indicative of three independent experiments.

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