TNF-α and IFN-s-Dependent Muscle Decay Is Linked to NF-𝜿B- and STAT-1α-Stimulated Atrogin1 and MuRF1 Genes in C2C12 Myotubes

Figure 3

Short- and long term effects of TNF-α, IFNα, IFNγ (10 ng/mL each), and insulin (10 nM) given alone, as pretreatment or together in the 3rd day of myogenesis. STAT-1α-P- Y⁷⁰¹ nuclear protein expression levels upon TNF-α and additional insulin pretreatment (a), STAT-1α transcriptional activity (b, d, f), Y⁷⁰¹-phospho-STAT-1α nuclear protein expression levels upon TNF-α and additional IFNα or IFNγ pretreatment (c), STAT-1α-P- Y⁷⁰¹ nuclear protein expression levels upon TNF-α and additional IFNα or IFNγ pretreatment, and subsequent insulin and IFNα or IFNγ cotreatment (e). Actin was used as loading control. The results are indicative of three independent experiments.