TNF-α and IFN-s-Dependent Muscle Decay Is Linked to NF-B- and STAT-1α-Stimulated Atrogin1 and MuRF1 Genes in C2C12 Myotubes
Figure 5
Changes in the cytosolic IκB and nuclear NF-κB expression levels in the 8th day of myogenesis (a). Long term effects of TNF-α, IFNα, IFNγ (10 ng/mL each), or metabolic inhibitors (curcumin 1 μM, AG 490 5 μM) given alone or together. Changes in the cytosolic myosin heavy chain IIa (fast form) expression levels in the 8th day of myogenesis (b). Actin was used as loading control. The results are indicative of three independent experiments.
