TNF-α and IFN-s-Dependent Muscle Decay Is Linked to NF-𝜿B- and STAT-1α-Stimulated Atrogin1 and MuRF1 Genes in C2C12 Myotubes

Figure 6

Changes in the cytosolic myosin heavy chain IIa (fast form), myogenin, and MyoD expression levels in selected days (1, 3, 5, 8) of 8 days of myogenesis from C2C12 myoblasts. Long term effects of TNF-α, IFNα, IFNγ (10 ng/mL each), or metabolic inhibitors (curcumin 1 μM, AG 490 5 μM) given alone or together. Actin was used as loading control. The results are indicative of three independent experiments.