TNF-α and IFN-s-Dependent Muscle Decay Is Linked to NF-B- and STAT-1α-Stimulated Atrogin1 and MuRF1 Genes in C2C12 Myotubes
Figure 7
Immunoprecipitation of STAT-1α shows time-dependent loss of immunoreactive TRADD in the precipitates upon TNF-α and/or IFNα or IFNγ cotreatment in the 8th day of myogenesis (a). IgG was used as equal input control. The results are indicative of three independent experiments. Long term effects (eight days) of TNF-α, IFNα, and IFN (10 ng/mL each) given alone or together on the Myhc IIa gene activity (b). Long term effects (eight days) of TNF-α, IFNα, IFNγ (10 ng/mL each, filled bars), or metabolic inhibitors (curcumin 1 μM) given alone (filled bars) or together (dotted bars) on Myhc IIa gene activity (c, d). Fold increase was calculated according to the formula described in Section 2.
