p38/AP-1 Pathway in Lipopolysaccharide-Induced Inflammatory Responses Is Negatively Modulated by Electrical Stimulation

Table 1

Sequences of primers used in semiquantitative and real-time PCR analyses.

Name		Sequence (5′ to 3′)
Real-time PCR
iNOS	F	GGA GCC TTT AGA CCT CAA CAG A
	R	TGA ACG AGG AGG GTG GTG
COX-2	F	GGGAGTCTGGAACATTGTGA
	R	GCACATTGTAAGTAGGTGGA
TNF-α	F	TGC CTA TGT CTC AGC CTC TT
	R	GAG GCC ATT TGG GAA CTT CT
GAPDH	F	CAA TGA ATA CGG CTA CAG CAA C
	R	AGG GAG ATG CTC AGT GTT GG
Semiquantitative RT-PCR
TNF-α	F	TTGACCTCAGCGCTGAGTTG
	R	CCTGTAGCCCACGTCGTAGC
IL-1β	F	CAGGATGAGGACATGAGCACC
	R	CTCTGCAGACTCAAACTCCAC
IL-6	F	GTACTCCAGAAGACCAGAGG
	R	TGCTGGTGACAACCACGGCC
COX-2	F	CACTACATCCTGACCCACTT
	R	ATGCTCCTGCTTGAGTATGT
iNOS	F	CCCTTCCGAAGTTTCTGGCAGCAGC
	R	GGCTGTCAGAGCCTCGTGGCTTTGG
IFN-β	F	CCACCACAGCCCTCTCCATCAACTAT
	R	CAAGTGGAGAGCAGTTGAGGACATC
IL-12	F	CAGAAGCTAACCATCTCCTGGTTTG
	R	TCCGGAGTAATTTGGTGCTTCACAC
GAPDH	F	CACTCACGGCAAATTCAACGGCAC
	R	GACTCCACGACATACTCAGCAC

F: forward, R: reverse.