Mediators of Inflammation / 2013 / Article / Fig 2

Research Article

Oxidized LDL Induces Alternative Macrophage Phenotype through Activation of CD36 and PAFR

Figure 2

oxLDL treatment inhibits LPS-induced NO, iNOS, and COX-2. Thioglycolate-elicited murine macrophages were treated with different concentrations of LDL or oxLDL for 24 h and then stimulated with LPS (1 μg/mL) for an additional 24 h. (a) Nitric oxide production was analyzed by the Griess assay. (b) iNOS and COX-2 expressions were analyzed by western blot, and the protein expression was quantified by AlphaEase FC software, V3.2 beta (Alpha Innotech). The autoradiographs show one representative experiment, *   versus LPS stimulated cells. (c) Cell viability was measured by MTT assay, * comparing oxLDL-treated with the nontreated cells. Data are presented as mean ± SEM of six independent experiments.

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