Oxidized LDL Induces Alternative Macrophage Phenotype through Activation of CD36 and PAFR
Engagement of PAFR and CD36 is required for the oxLDL-induced M2 phenotype. Murine BMDM were pretreated with PAFR antagonists WEB2086 (50 μM) or CV3988 (10 μM) alone or in combination with blocker antibody to CD36 (1 : 500) for 30 min and then treated with oxLDL (20 μg/mL). (a) The mRNA expression of TGF-β, Arg-1, RANTES and CXCL2 was analyzed by real-time PCR after 5 h. (b) BMDM were treated overnight with oxLDL and then stimulated with LPS (10 ng/mL). IL-10 and IL-12 production was assessed in the supernatants by ELISA assays. * comparing oxLDL-treated with nontreated cells and # versus oxLDL-treated cells.
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