Regulation of Regenerative Periodontal Healing by NAMPT
Figure 3
Effect of EMD on the RUNX2 (a) mRNA expression in the presence and absence of NAMPT (100 ng/mL) at 1 d and 3 d. Untreated cells served as control. All experiments were performed in triplicate and repeated at least twice. Mean ± SEM (); *significant () difference between groups. Effect of EMD on the calcium accumulation in the presence and absence of NAMPT (100 ng/mL) in cultures of PDL cells ((b) and (c)). Untreated cells served as control. The calcium accumulation was analysed by alizarin red S staining, visualized by microscopy (c), and quantified by elution with cetylpyridinium chloride (b) at 14 d. All experiments were performed in triplicate and repeated at least twice. Mean ± SEM (); *significant () difference between groups. Images from one representative donor are shown.
